rabbit anti psyk y352 65e4 id catalog Search Results


rabbit anti psyk y352 65e4 id catalog  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit anti psyk y352 65e4 id catalog
(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
Rabbit Anti Psyk Y352 65e4 Id Catalog, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+psyk+y352+65e4+id+catalog/Phospho-Zap-70+(Tyr319)%2FSyk+(Tyr352)+Rabbit+mAb/bio_rxiv__2025__04__16__648985-179-7-4
Average 95 stars, based on 1 article reviews
rabbit anti psyk y352 65e4 id catalog - by Bioz Stars, 2026-09
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cat 13198s rrid ab 2687924 phospho syk y352  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc cat 13198s rrid ab 2687924 phospho syk y352
(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
Cat 13198s Rrid Ab 2687924 Phospho Syk Y352, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
cat 13198s rrid ab 2687924 phospho syk y352 - by Bioz Stars, 2026-09
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rabbit anti human p21 n 20  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology rabbit anti human p21 n 20
(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
Rabbit Anti Human P21 N 20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti human p21 n 20 - by Bioz Stars, 2026-09
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rabbit anti human bid  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti human bid
(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
Rabbit Anti Human Bid, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+psyk+y352+65e4+id+catalog/BID+Antibody/pmc05909974-96-57-61
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rabbit anti human bid - by Bioz Stars, 2026-09
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rabbit anti human lck  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc rabbit anti human lck
(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
Rabbit Anti Human Lck, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+psyk+y352+65e4+id+catalog/Lck+Antibody/pmc05909974-53-28-61
Average 94 stars, based on 1 article reviews
rabbit anti human lck - by Bioz Stars, 2026-09
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rabbit anti human syk  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti human syk
(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
Rabbit Anti Human Syk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+psyk+y352+65e4+id+catalog/Syk+Antibody/pmc05909974-53-39-61
Average 96 stars, based on 1 article reviews
rabbit anti human syk - by Bioz Stars, 2026-09
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santa cruz biotechnology cat  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology santa cruz biotechnology cat
(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
Santa Cruz Biotechnology Cat, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+psyk+y352+65e4+id+catalog/Cbl-b+Antibody/pm38019655-569-124-124
Average 94 stars, based on 1 article reviews
santa cruz biotechnology cat - by Bioz Stars, 2026-09
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4970l rrid ab 2223172 cbl monoclonal rabbit d4e10 cell signaling technology  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc 4970l rrid ab 2223172 cbl monoclonal rabbit d4e10 cell signaling technology
(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
4970l Rrid Ab 2223172 Cbl Monoclonal Rabbit D4e10 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+psyk+y352+65e4+id+catalog/beta-Actin+Rabbit+mAb/pm38019655-569-81-87
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2710s rrid ab 2197222 phospho syk y525  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc 2710s rrid ab 2197222 phospho syk y525
(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
2710s Rrid Ab 2197222 Phospho Syk Y525, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+psyk+y352+65e4+id+catalog/Phospho-Syk+(Tyr525%2F526)+Rabbit+mAb/pm38019655-569-62-68
Average 95 stars, based on 1 article reviews
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cat 8869s rrid ab 11217636 phospho cbl y774 polyclonal rabbit cell signaling technology  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc cat 8869s rrid ab 11217636 phospho cbl y774 polyclonal rabbit cell signaling technology
(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
Cat 8869s Rrid Ab 11217636 Phospho Cbl Y774 Polyclonal Rabbit Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cat 3555s rrid ab 2070451 phospho cbl y731 polyclonal rabbit cell signaling technology  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc cat 3555s rrid ab 2070451 phospho cbl y731 polyclonal rabbit cell signaling technology
(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
Cat 3555s Rrid Ab 2070451 Phospho Cbl Y731 Polyclonal Rabbit Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cat 3554s rrid ab 2070452 cbl b monoclonal mouse  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc cat 3554s rrid ab 2070452 cbl b monoclonal mouse
(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.
Cat 3554s Rrid Ab 2070452 Cbl B Monoclonal Mouse, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk Y352 (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.

Journal: bioRxiv

Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse

doi: 10.1101/2025.04.16.648985

Figure Lengend Snippet: (A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk Y352 (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.

Article Snippet: Antibodies were obtained from Cell Signaling Technologies (rabbit anti-pSyk Y352 65E4 ID/catalog # (#)2717, rabbit anti-pPLCγ Y1217 #3871, rabbit anti-pSHIP1 Y1020 #3941, rabbit anti-pSHP-1 Y564 D11G5 #8849, rabbit anti-pErk1/2 T202/Y204 D13.14.4e #4370, rabbit anti-pAkt S473 193H12 #4058, rabbit anti-pPI3K p85(Y458)/p55(Y199) #4228, mouse anti-β-Actin 8H10D10 #3700), Abcam (Cambridge, United Kingdom) (mouse anti-LynA+B Lyn01 ab1890), Santa Cruz (Dallas, TX, USA) (rabbit anti-LynA+B 44 #sc-15, goat anti-Hck M-28 #sc-1428), and LICOR (donkey anti-mouse IgG 800CW #925-32212, donkey anti-rabbit IgG 800CW #925-32213, donkey anti-mouse IgG 680LT #925-68022, donkey anti-goat IgG 680LT #926-32214 ) .

Techniques: Staining, Fluorescence

Densitometry quantification of protein species in immunoblotted lysates from IFN-γ-primed Csk AS BMDMs after 5 min treatment with 3-IB-PP1 or zym dep probed by immunoblot for (A) pSyk Y352 , (B) pPLCγ2 Y1217 , (C) pPI3K p85/p55 (D) immunoprecipitated Ras-GTP, (E) pErk1/2 Y202/T204 , and (F) pAkt S473 . Error: SEM; n=9-14 for 3-IB-PP1 and 11-15 for zym dep . Sig. assessed via non-parametric t test and Kolmogorov-Smirnov test: * P = 0.0216, *** P = 0.0007, ** P < 0.0001.

Journal: bioRxiv

Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse

doi: 10.1101/2025.04.16.648985

Figure Lengend Snippet: Densitometry quantification of protein species in immunoblotted lysates from IFN-γ-primed Csk AS BMDMs after 5 min treatment with 3-IB-PP1 or zym dep probed by immunoblot for (A) pSyk Y352 , (B) pPLCγ2 Y1217 , (C) pPI3K p85/p55 (D) immunoprecipitated Ras-GTP, (E) pErk1/2 Y202/T204 , and (F) pAkt S473 . Error: SEM; n=9-14 for 3-IB-PP1 and 11-15 for zym dep . Sig. assessed via non-parametric t test and Kolmogorov-Smirnov test: * P = 0.0216, *** P = 0.0007, ** P < 0.0001.

Article Snippet: Antibodies were obtained from Cell Signaling Technologies (rabbit anti-pSyk Y352 65E4 ID/catalog # (#)2717, rabbit anti-pPLCγ Y1217 #3871, rabbit anti-pSHIP1 Y1020 #3941, rabbit anti-pSHP-1 Y564 D11G5 #8849, rabbit anti-pErk1/2 T202/Y204 D13.14.4e #4370, rabbit anti-pAkt S473 193H12 #4058, rabbit anti-pPI3K p85(Y458)/p55(Y199) #4228, mouse anti-β-Actin 8H10D10 #3700), Abcam (Cambridge, United Kingdom) (mouse anti-LynA+B Lyn01 ab1890), Santa Cruz (Dallas, TX, USA) (rabbit anti-LynA+B 44 #sc-15, goat anti-Hck M-28 #sc-1428), and LICOR (donkey anti-mouse IgG 800CW #925-32212, donkey anti-rabbit IgG 800CW #925-32213, donkey anti-mouse IgG 680LT #925-68022, donkey anti-goat IgG 680LT #926-32214 ) .

Techniques: Western Blot, Immunoprecipitation

(A-D) Immunoblots and quantifications of lysates from IFN-γ-primed Lyn +/+ and Lyn KO BMDMs probed for phosphorylated (A) Syk Y352 , (B) Erk1/2 T202/Y204 , (C) Akt S473 , and (D) SHIP1 Y1020 . β-Actin is a visual loading control, and quantifications were normalized to total protein content in the gel lane. Data are shown relative to the signal in Lyn +/+ . n=10-15 for Lyn +/+ and 6-7 for Lyn KO . Error bars: SEM. Sig. nonparametric, unpaired t with Kolmogorov-Smirnov test, *P=0.0152, **P≥0.007, ***P=0.0006 and ****P≤0.0001. (E) Representative images of PIP 3 (yellow) and nuclear staining (cyan) in IFN-γ -primed BMDMs. (F) Quantification of the geometric mean fluorescence intensity (gMFI) of PIP 3 staining in Lyn KO relative to Lyn +/+ BMDMs. Data points reflect the mean and SEM of 3-4 independent wells from 4 biological replicates. Sig. nonparametric, unpaired t test with Kolmogorov-Smirnov test, *** P<0.0006.

Journal: bioRxiv

Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse

doi: 10.1101/2025.04.16.648985

Figure Lengend Snippet: (A-D) Immunoblots and quantifications of lysates from IFN-γ-primed Lyn +/+ and Lyn KO BMDMs probed for phosphorylated (A) Syk Y352 , (B) Erk1/2 T202/Y204 , (C) Akt S473 , and (D) SHIP1 Y1020 . β-Actin is a visual loading control, and quantifications were normalized to total protein content in the gel lane. Data are shown relative to the signal in Lyn +/+ . n=10-15 for Lyn +/+ and 6-7 for Lyn KO . Error bars: SEM. Sig. nonparametric, unpaired t with Kolmogorov-Smirnov test, *P=0.0152, **P≥0.007, ***P=0.0006 and ****P≤0.0001. (E) Representative images of PIP 3 (yellow) and nuclear staining (cyan) in IFN-γ -primed BMDMs. (F) Quantification of the geometric mean fluorescence intensity (gMFI) of PIP 3 staining in Lyn KO relative to Lyn +/+ BMDMs. Data points reflect the mean and SEM of 3-4 independent wells from 4 biological replicates. Sig. nonparametric, unpaired t test with Kolmogorov-Smirnov test, *** P<0.0006.

Article Snippet: Antibodies were obtained from Cell Signaling Technologies (rabbit anti-pSyk Y352 65E4 ID/catalog # (#)2717, rabbit anti-pPLCγ Y1217 #3871, rabbit anti-pSHIP1 Y1020 #3941, rabbit anti-pSHP-1 Y564 D11G5 #8849, rabbit anti-pErk1/2 T202/Y204 D13.14.4e #4370, rabbit anti-pAkt S473 193H12 #4058, rabbit anti-pPI3K p85(Y458)/p55(Y199) #4228, mouse anti-β-Actin 8H10D10 #3700), Abcam (Cambridge, United Kingdom) (mouse anti-LynA+B Lyn01 ab1890), Santa Cruz (Dallas, TX, USA) (rabbit anti-LynA+B 44 #sc-15, goat anti-Hck M-28 #sc-1428), and LICOR (donkey anti-mouse IgG 800CW #925-32212, donkey anti-rabbit IgG 800CW #925-32213, donkey anti-mouse IgG 680LT #925-68022, donkey anti-goat IgG 680LT #926-32214 ) .

Techniques: Western Blot, Control, Staining, Fluorescence

Immunoblots and quantifications of (A-B) pSyk Y352 , (C-D) pErk1/2 T202/Y204 , (E-F) pAkt S473 , and (G-H) pSHIP1 Y1020 in IFN-γ-primed Csk AS (Lyn +/+ ) or Csk AS Lyn KO (Lyn KO ) BMDMs treated with (A, C, E, G) 3-IB-PP1 or (B, D, F, H) zym dep , corrected for total protein in each gel lane and shown relative to t=0 of Lyn +/+ ; n=10-11 for Lyn +/+ , 4-7 for Lyn KO . Error bars: SEM. Sig. via two-way ANOVA with Sidak’s multiple comparisons test: * P = 0.0143, ** P = 0.0041, **** P<0.0001.

Journal: bioRxiv

Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse

doi: 10.1101/2025.04.16.648985

Figure Lengend Snippet: Immunoblots and quantifications of (A-B) pSyk Y352 , (C-D) pErk1/2 T202/Y204 , (E-F) pAkt S473 , and (G-H) pSHIP1 Y1020 in IFN-γ-primed Csk AS (Lyn +/+ ) or Csk AS Lyn KO (Lyn KO ) BMDMs treated with (A, C, E, G) 3-IB-PP1 or (B, D, F, H) zym dep , corrected for total protein in each gel lane and shown relative to t=0 of Lyn +/+ ; n=10-11 for Lyn +/+ , 4-7 for Lyn KO . Error bars: SEM. Sig. via two-way ANOVA with Sidak’s multiple comparisons test: * P = 0.0143, ** P = 0.0041, **** P<0.0001.

Article Snippet: Antibodies were obtained from Cell Signaling Technologies (rabbit anti-pSyk Y352 65E4 ID/catalog # (#)2717, rabbit anti-pPLCγ Y1217 #3871, rabbit anti-pSHIP1 Y1020 #3941, rabbit anti-pSHP-1 Y564 D11G5 #8849, rabbit anti-pErk1/2 T202/Y204 D13.14.4e #4370, rabbit anti-pAkt S473 193H12 #4058, rabbit anti-pPI3K p85(Y458)/p55(Y199) #4228, mouse anti-β-Actin 8H10D10 #3700), Abcam (Cambridge, United Kingdom) (mouse anti-LynA+B Lyn01 ab1890), Santa Cruz (Dallas, TX, USA) (rabbit anti-LynA+B 44 #sc-15, goat anti-Hck M-28 #sc-1428), and LICOR (donkey anti-mouse IgG 800CW #925-32212, donkey anti-rabbit IgG 800CW #925-32213, donkey anti-mouse IgG 680LT #925-68022, donkey anti-goat IgG 680LT #926-32214 ) .

Techniques: Western Blot

Immunoblots and quantification of (A) pSHIP1 Y1020 , (B) pAkt S473 , (C) pSyk Y352 , and (D) pErk T202/Y204 in CskAS (Hck +/+ Fgr +/+ ) and Csk AS Hck KO Fgr KO (Hck KO Fgr KO ) BMDMs, corrected for the total protein in each lane and shown relative to t=0 of Hck +/+ Fgr +/+ . Error bars: SEM, n=10-11 for Hck +/+ Fgr +/+ and n=4 for Hck KO Fgr KO . Sig. 2-way ANOVA with Sidak’s multiple comparisons test: * P = 0.0499.

Journal: bioRxiv

Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse

doi: 10.1101/2025.04.16.648985

Figure Lengend Snippet: Immunoblots and quantification of (A) pSHIP1 Y1020 , (B) pAkt S473 , (C) pSyk Y352 , and (D) pErk T202/Y204 in CskAS (Hck +/+ Fgr +/+ ) and Csk AS Hck KO Fgr KO (Hck KO Fgr KO ) BMDMs, corrected for the total protein in each lane and shown relative to t=0 of Hck +/+ Fgr +/+ . Error bars: SEM, n=10-11 for Hck +/+ Fgr +/+ and n=4 for Hck KO Fgr KO . Sig. 2-way ANOVA with Sidak’s multiple comparisons test: * P = 0.0499.

Article Snippet: Antibodies were obtained from Cell Signaling Technologies (rabbit anti-pSyk Y352 65E4 ID/catalog # (#)2717, rabbit anti-pPLCγ Y1217 #3871, rabbit anti-pSHIP1 Y1020 #3941, rabbit anti-pSHP-1 Y564 D11G5 #8849, rabbit anti-pErk1/2 T202/Y204 D13.14.4e #4370, rabbit anti-pAkt S473 193H12 #4058, rabbit anti-pPI3K p85(Y458)/p55(Y199) #4228, mouse anti-β-Actin 8H10D10 #3700), Abcam (Cambridge, United Kingdom) (mouse anti-LynA+B Lyn01 ab1890), Santa Cruz (Dallas, TX, USA) (rabbit anti-LynA+B 44 #sc-15, goat anti-Hck M-28 #sc-1428), and LICOR (donkey anti-mouse IgG 800CW #925-32212, donkey anti-rabbit IgG 800CW #925-32213, donkey anti-mouse IgG 680LT #925-68022, donkey anti-goat IgG 680LT #926-32214 ) .

Techniques: Western Blot